Catalog Number - 62508-15P-2

Hyper Similars C8™ Octyl is a traditional reversed-phase HPLC stationary phase manufactured on high-purity Type-B spherical silica with a fully endcapped octyl (C8) bonded phase. Designed as a cost-effective alternative to legacy Hypersil® and Spherisorb® columns, it offers comparable chromatographic behavior for USP L7 methods while providing the moderate hydrophobic retention and reproducibility expected from a conventional C8 stationary phase. Compared to C18 columns, C8 phases generally provide reduced retention and faster elution of hydrophobic analytes.

62508-15P-2 is a 150 mm x 2.1 mm ID narrow-bore analytical column packed with 5 µm, 150Å Hyper Similars C8 particles. The 150 mm length provides increased resolving power compared to shorter columns, making it useful for applications requiring additional separation. The 2.1 mm ID format minimizes solvent consumption and is optimized for LC-MS workflows. Typical operating flow rates are approximately 0.2–0.3 mL/min and require an HPLC or LC-MS system capable of reliable low-flow operation with low-volume flow cells and plumbing.

This USP L7 column is ideal for hydrophobic and neutral compounds when less retention is desired than a comparable C18 phase. Common applications include LC-MS methods, pharmaceutical analysis, long-chain fatty acid separations, and method development where enhanced sensitivity and additional chromatographic resolution are important.

Key Benefits

• Type-B spherical silica stationary phase

• Suitable alternative for USP L7 methods

• Comparable selectivity to Hypersil® and Spherisorb® C8 columns

• Narrow-bore format ideal for LC-MS applications

• Increased resolution from 150 mm column length

• Lower retention than C18 columns

• Reduced solvent consumption

• End-capped C8 bonded phase

Typical Applications

• USP L7 methods

• LC-MS analysis

• Reversed-phase HPLC

• Pharmaceutical analysis

• Long-chain fatty acid analysis

• Method development

• Quality control testing

• Method transfer and validation

Specifications

• Product Type: HPLC Column

• Stationary Phase: Hyper Similars C8 Octyl

• USP Classification: L7

• Particle Size: 5 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: C8 (Octyl)

• Endcapped: Yes

• Pore Size: 150Å

• Column Dimensions: 2.1 mm ID x 150 mm

• Length: 150 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Narrow-bore format optimized for LC-MS applications

• Requires an HPLC or LC-MS system capable of reliable low-flow operation

• Lower operating pressure than comparable 3 µm columns

• Reduced solvent consumption compared to 4.6 mm ID columns

• Provides less retention than comparable C18 stationary phases

• Increased resolving power for complex separations

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